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| De Conti, Daniela; Corredor-Prado, Jenny Paola; Roecker Junior, Djalma; Suzuki, Rogério Mamoru; Guerra, Miguel Pedro; Pescador, Rosete. |
| Tissue culture techniques have been employed for orchid mass propagation by means of the morphogenetic route of protocorm like-bodies (PLBs). This study aimed to analyze and compare Indole-acetic acid (IAA) and sugar endogenous levels in protocorm-like bodies (PLBs) induction and development in Cattleya tigrina, in order to better understand this process and to optimize micropropagation procedure protocols. Leaves grown on MS (Murashige and Skoog) culture medium, suplemented with 9 μM TDZ for PLBs induction and development were collected after 0, 2, 7, 14, 30, 60 and 100 days of cultivation, for further analysis. Increase of IAA and reduction of sugar levels are strongly related to morphogenetic response, that is, PLBs formation over the preexisting ones... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Orchid; Hormones; Sugars; Morphogenesis.. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37874 |
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| Corredor, Jenny; De Conti, Daniela; Guerra, Miguel Pedro; Dal Vesco, Lirio Luiz; Pescador, Rosete. |
| Tissue culture techniques have been employed for bromeliad mass propagation by means of the morphogenetic route of nodular cluster cultures (NCs). This study aimed to assess protein, carbohydrate and global DNA methylation (GDM) level dynamics during NCs induction from Vriesea reitzii seeds. Seeds were inoculated into Murashige and Skoog (MS) liquid medium supplemented with 4 µM α-naphthaleneacetic acid (NAA) to induce NCs and in culture medium without plant growth regulators to form normal seedlings. Samples collected at 0, 3, 7, 10, 14, and 21 days of culture were analyzed. All parameters assessed showed the same variation pattern. However, seeds inducing NCs showed significantly lower starch (6.0 mg g-1 FM), carbohydrate (10.7 mg g-1 FM) and GDM (11.0%)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bromeliaceae; Bromeliad; Germination; Micropropagation; Seedling. Bromeliaceae bromeliad germination micropropagation seedling.. |
| Ano: 2020 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42448 |
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